High-Resolution Imaging

Microscopy is an essential technique in the life science and developments in microscopy have almost always led to new biological discoveries. In the course "High Resolution Imaging" we introduce the state of the art techniques to observe cells, tissue, and biological molecules at the highest possible resolution. We provide a working knowledge of various high resolution imaging techniques, present the basic principles and limitations behind these techniques, and also discuss how to prepare biological samples for the different forms of microscopy. Examples from recent scientific literature will be used to illustrate what can be and has been achieved. Targeted homework, lab demonstrations, and experimental as well as theoretical exercises will help to familiarize you with the different types of microscopes and the data they provide.

Specifically, the course includes lectures and demos on:

• Superresolution fluorescence microscopy.

• Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), and Scanning transmission electron microscopy (STEM).

• Electron cryo-microscopy, and methods to calculate 3D structures from 2D projections.

• Correlative light and electron microscopy and techniques for large-scale and volume imaging.

1. The assessment consists of assignments and a written exam.

1.a the written exam is scheduled for week 1.10

2. The resit consists of a written exam. The resit may be an oral exam if fewer than 5 students enroll for the retake.

2.a the resit of the written exam is scheduled for week 2.10

3. There is no opportunity to repair an individual assignment grade.
4. The final grade is composed as follows. Each part must be passed with a minimum of 5.5 to receive a passing grade.

Written exam (or oral): 70%

Assignments:  30%

Partial grades are only good for the year that they’re earned and do not carry over to the next year.
5. Registration for exams is done via Osiris. Submitting assignments via the course Brightspace by their deadline will get them graded and included in the final grade. 

6. Students needing to turn work in late due to personal circumstances must contact the Academic Counsellor prior to turning it in to make arrangements. Other late work might not be graded.