Molecular Biotechnology and Genomics

The master’s-level course LM3601 Molecular Biotechnology and Genomics offers an in-depth exploration of the molecular mechanisms underlying biological systems, complemented by hands-on training in the advanced techniques that drive innovation across the life sciences. Students will explore and apply state-of-the-art tools and approaches to engineer modern microbial cell factories, including next-generation cloning, synthetic biology, metabolic engineering, and CRISPR/Cas genome editing.

Emphasis will be placed on cutting-edge genomics technologies, such as next-generation sequencing and transcriptomics, as well as the computational methods used to analyze and interpret large-scale sequencing data. The course will highlight practical applications in microbial biotechnology and sustainable bioproduction. Through lectures, literature analysis, assignments, and hands-on exercises, students will learn to design synthetic gene circuits, optimize metabolic pathways for industrial fermentation, and leverage molecular tools to create novel bioproducts.

By the end of the course, students will have gained a strong theoretical foundation and practical skill set that will prepare them for careers in R&D, biotechnology start-ups, and other innovation-driven sectors where molecular biology and genomics play a central role.

1- Molecular Biology and genetics

• Cloning strategies

• Deletion, silencing, overexpression

• Vector design

• Restriction versus recombination

• Strain design

  • integration HR vs NHEJ

2- Genomics

• Sequencing industrial organism, why?

• Methods from Sanger to next generation sequencing techniques

• Analysis of genome sequences

  • de novo genome sequence assembly

Students are assessed using three different methods:

1. Individual Assignments (45% of the final grade)

   • Two assignments are given during the first period of the course. Students have 2–3 weeks to complete each one and must submit their work by a clearly specified deadline. Each of these assignments accounts for 15% of the final grade.

   • A third assignment is completed at the end of the second period during a supervised in-class session (3.5 hours). This assignment focuses on genomics content and is designed to evaluate the students’ ability to apply concepts related to genome sequence analysis and assembly. This assignment also accounts for 15% of the final grade.

2. Final Exam (55% of the final grade)

   • The course concludes with a closed-book written exam that tests students’ understanding of all key concepts covered in the lectures and practical sessions.

All grades are assigned on an individual basis.