Molecular Biotechnology and Genomics

The master’s-level course LM3601 Molecular Biotechnology and Genomics offers an in-depth exploration of the molecular mechanisms underlying biological systems, complemented by hands-on training in the advanced techniques that drive innovation across the life sciences. Students will explore and apply state-of-the-art tools and approaches to engineer modern microbial cell factories, including next-generation cloning, synthetic biology, metabolic engineering, and CRISPR/Cas genome editing.

Emphasis will be placed on cutting-edge genomics technologies, such as next-generation sequencing and transcriptomics, as well as the computational methods used to analyze and interpret large-scale sequencing data. The course will highlight practical applications in microbial biotechnology and sustainable bioproduction. Through lectures, literature analysis, assignments, and hands-on exercises, students will learn to design synthetic gene circuits, optimize metabolic pathways for industrial fermentation, and leverage molecular tools to create novel bioproducts.

By the end of the course, students will have gained a strong theoretical foundation and practical skill set that will prepare them for careers in R&D, biotechnology start-ups, and other innovation-driven sectors where molecular biology and genomics play a central role.

1- Molecular Biology and genetics

• Cloning strategies

• Deletion, silencing, overexpression

• Vector design

• Restriction versus recombination

• Strain design

  • integration HR vs NHEJ

2- Genomics

• Sequencing industrial organism, why?

• Methods from Sanger to next generation sequencing techniques

• Analysis of genome sequences

  • de novo genome sequence assembly